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Abstract
A Simple, accurate and rapid RP-HPLC method has been developed for the estimation of ritonavir (RIT) in bulk and pharmaceutical dosage forms using a hypersil BDS C 18, 100 x 4.6 mm i.d, 5 µm particle size in isocratic mode; with mobile phase comprising of buffer (0.02M potassium dihydrogen phosphate) and acetonitrile in the ratio 70:30 (v/v).The flow rate was 1 ml/min and detection was carried out by photodiode array detector at 237 nm. The retention time for RIT was found to be 2.55 min. the proposed method has permitted the quantification of RIT over linearity in the range of 25 – 150 µg/ml and its percentage recovery was found to be 99.299 – 100.575 %. The % RSD of intraday and inter day precision were found 0.37% and 0.4% according to International Conference on Harmonization (ICH) Q2B guidelines.