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Abstract

Dried and powder rhizome of Acoruscalamus (Araceae) was used to isolate the chemical constituent using phase column chromatography and TLC purification. Structure was elucidated by spectroscopic methods. For the evaluation of antistress activity, groups of rats (n = 6) were subjected to forced swim stress one hour after daily treatment of A. calmusextract. Urinary vanillylmandelic acid (VMA) and ascorbic acid were selected as non-invasive biomarkers to assess the antistress activity. The 24 h urinary excretion of vanillylmandelic acid (VMA) and ascorbic acid were determined by spectrophotometric methods in all groups under normal and stressed conditions. No change in the urinary excretion of VMA and ascorbic acid was observed in normal animals at all the doses studied.

Keywords

Adaptogenic activity Stress Acoruscalamus Vanillylmandelic acid Ascorbic acid

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