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Abstract
The objective of the present investigation was to isolate the active components present in whole plant of .. The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), methanol was found to be more active among them. The preliminary phytochemical results revealed that flavonoids and amino acids as active constituents in methanolic extract of Teramnuslabialis.Themethanolic extract of Teramnuslabialis was undergone column chromatography with different solvent fractions. Hence, two compounds were isolated from methanolic extract of Teramnuslabialis with the compound 1 was eluted with Fraction 8-11 (eluted petroleum ether – Benzene 70:30), gave a solid designated as compound 1 (100 mg) and fraction 40 – 43 (eluted Benzene – ethyl acetate 10:90, gave a solid designated as compound 2 (150 mg) and fraction 62-67 (eluted ethyl acetate – methanol 90:10) gave a solid designated as compound 3 (162 mg). The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as 5, 6 Dihydro – 6 dodekylpyran – 2 one and its molecular formula is deduced as C17 H28 O2; Structure of compound 2 is proposed as to 4’ 5, 6’ – Trihydroxy – 7-methoxy- isoflavanone – 2 carboxylic acid and its molecular formula is deduced as C17 H12 O8.Therefore, further biological investigations need to be carried out isolated compounds present in this plant.