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Abstract
Screening of medicinal plants for bioactive compounds leads to less expensive new antimicrobial agents with improved safety & efficacy. Azadirachta indica (Neem) is a multipurpose tree with multipurpose tree multiple health benefits. Different parts of the plants are shown to exhibit antimicrobial effects against a wide variety of microorganism. In present study we compared the anti-microbial efficacy of aqueous extracts of seeds of a Azadirachta indica human pathogenic bacteria (Staphylococcus aureus, Escherichia coli pneumonia and skin disease are some types of severe infections occur worldwide. Due to pathogenic bacteria such as Escherichia coli(E.coli) Staphylococcus aureus (S. aureus). These bacteria’s do contribute towards other infection in human and thus lead to towards unhealthy life. Agar well diffusion method and micro-broth dilution methods were used to determine the minimum inhibitory concentration(MIC). Results showed that seed extracts exhibited strong anti-microbial activity against bacteria at all the concentration tested (100,200 & 300 g/ml). Our results suggest that aqueous extracts of Azadirachta indica seed exhibit high anti-microbial activity.