New Analytical Method Development And Validation For The Determination Of Paclitaxel In Bulk Drug And Formulation By Rp-Hplc

Abstract

A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Paclitaxel, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 250mm, 5µm) column using a mixture of Acetonitrileand Water (50:50% v/v)as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 285nm. The retention time of the Paclitaxelwas 3.0±0.02min. The method produces linear responses in the concentration range of 10-50ppm of Paclitaxel. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.