Main Article Content
Abstract
Aim
The aim of the present research work was to develop a sensitive, rapid and accurate, RP-UPLC method for the simultaneous estimation of voxilaprevir (VXR), sofosbuvir (SFR), and velpatasvir (VLR) in formulations.
Materials and methods
The chromatographic separation of mixture of VXR, SFR, and VLR was attained in isocratic method utilizing a mobile phase of 0.01N potassium dihydrogen orthophosphate (pH 4.8) and methanol in the proportion of 50:50% v/v utilizing a CHS C18 column which has dimensions of 100×2.1mm,2.0 µmparticlesizeandtheflowrateof1.0mL/min.Thedetectionsystemwasmonitoredat260nm wavelength maximum with
1.0 mL injection volume.
Results
The retaining time for VXR, SFR, and VLR was achieved at 1.468 min, 0.606 min, and 0.848 min, respectively.
Conclusion
The developed method was highly ensitive, rapid, precise, and accurate than the earlier reported methods. The total run time was decreased to 3.0 min; hence, the technique was more precise and economical. The projected method can be utilized for routine analysis in quality control department in pharmaceutical companies.