Analytical method development and validation for the simultaneous estimation of doxepin by using rp-hplc technique

Abstract

A simple and selective LC method is described for the determination of Doxepin dosage forms. Chromatographic separation was achieved on a c₁₈ column using mobile phase consisting of a mixture of Triethyl amine: ACN (60:40v/v/v), with detection of 250 nm. Linearity was observed in the range 75-150 µg /ml for Doxepin(r² =0.999) for drugs estimated by the proposed methods was in good agreement with the label claim quality control department in industries, approved testing laboratories, bio-pharmaceutical and bio-equivalence studies and in clinical pharmacokinetic studies in near future.