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Abstract
Sesbaniagrandiflora, a plant in family Leguminosae is found widely in Thailand. The leaf and flower of the plants has been utilized as food whereas its stem has been used as a traditional medicine. Sesbaniagrandiflora. Linn Leaves were broadly used in treatment of several diseases like CNS depressant, analgesic [1], anti-oxidant [2], microbial infections [3]etc. However, there is very few scientific data on biological activity of this plant. In the present study, the leaves of Sesbaniagrandiflora L. were successively extracted with n-hexane and Ethyl acetate (EA) by soxhlet extraction. The EA extract was dried and preliminary phytochemical screening was done, which revealed the presence of alkaloid, tannins, flavanoids, steroids and glycosides. Acute toxicity study was carried out using the fixed dose method according to OECD guideline no. 423. The different doses like 500, 1000, 2000, 3000 and 4000 mg/Kg body weight were administered orally to the animals, observed for 24 hr after dosing and also observed for 14 days without giving drug. EA extract was given to rabbits in 200 mg/kg and 400 mg/kg with Disulfiram as standard. Both the dose levels of extracts exhibited significant inhibition of Aldehyde dehydrogenase enzyme there by the elevated ketone bodies in rabbit serum was observed. The inhibition of aldehyde dehydrogenase by the extract was found to be less potent than the standard Disulfiram.