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Abstract
The aim of the present work was to develop a gradient RP-HPLC for simultaneous analysis of Doxycycline and Tinidazole in tablet dosage form. Method: chromatographic system was optimized using an agilent XDB C18 (150 x 4.6mm,5µm) column with potassium dihydrogen phosphate ( pH 3) and acetonitrile in the ratio of 70;30%v/v, as a mobile phase, at a flow rate of 1.0 ml/min. Detection was carried out at 220nm by a photodiode array detector. Result: doxycycline and tinidazole were eluted with retention times of 3.372 and 2.490 mins, respectively. Beer’s lambert’s law was obeyed over the concentration ranges of 50 -200µg/ml and 150 - 600µg/ml for doxycycline and tinidazole, respectively. Conclusion: the high recovery and low coefficients of variation confirm the suitability of the method for simultaneous analysis of both drugs in a tablet dosage form. Statistical analysis proves that the method is sensitive and significant for the analysis of doxycycline and tinidazole in pure and in pharmaceutical dosage form without any interference from the excipients. The method was validated in accordance with ICH guidelines. Validation revealed the method is specific, rapid, accurate, precise, reliable, and reproducible.